ROS assay cell type

Get tips on using Eco52I (XmaIII) restriction enzyme to perform Restriction Enzymes Eco52I / EagI

Get tips on using Bsp1286I (SduI) restriction enzyme to perform Restriction Enzymes Bsp1286I / SduI

Get tips on using SmiI (SwaI) restriction enzyme to perform Restriction Enzymes SwaI / SmiI

Get tips on using BssHII (BsePI) restriction enzyme to perform Restriction Enzymes BssHII / PteI

Get tips on using HincII (HindII) restriction enzyme to perform Restriction Enzymes HincII / HindII

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using PE anti-mouse CD49b (pan-NK cells) Antibody to perform Flow cytometry Anti-bodies Mouse - CD49b

Get tips on using E.Z.N.A.® Total RNA Kit I to perform RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells