Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse

Get tips on using Insulin (Autostainer Link 48) to perform Immunohistochemistry Mouse - Insulin

Get tips on using Lysozyme EC 3.2.1.17 (Concentrate) to perform Immunohistochemistry Mouse - Lysozyme

Get tips on using Ki-67 Antigen, Clone MIB-1 to perform Immunohistochemistry Ki67 - Rabbit Mouse / Human -NA-

Get tips on using Rat GFR alpha-1/GDNF R alpha-1 Antibody to perform Immunohistochemistry Mouse - GFRA1

Get tips on using Anti-CD105 antibody [MJ7/18] (PE) (ab93567) to perform Flow cytometry Anti-bodies Mouse - CD105

Get tips on using Insulin (C27C9) Rabbit mAb #3014 to perform Immunohistochemistry Mouse - Insulin

Get tips on using Anti-Integrin β1 Antibody, clone MB1.2 to perform Flow cytometry Anti-bodies Mouse - CD29/β1-Integrin

Get tips on using Recombinant Anti-CD204/MSR1 Antibody (FITC), Rabbit Monoclonal to perform Flow cytometry Anti-bodies Mouse - CD204

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.