Site Directed Mutagenesis (SDM) Human Point mutation HeLa

Get tips on using Accell Human VEGFC (7424) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LAMA84 VEGFC

Get tips on using Accell Human MYB (4602) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - K562 MYB

Get tips on using Accell Human VEGFC (7424) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - K562 VEGFC

Get tips on using Accell Human CD44 (960) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HaCaT CD44

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Accell Human MYB (4602) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MEG-01 MYB

Get tips on using Accell Human VEGFC (7424) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MEG-01 VEGFC

Get tips on using Accell Human MYB (4602) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - EM-2 MYB

Get tips on using Accell Human VEGFC (7424) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - EM-2 VEGFC

Get tips on using Human ShhN ELISA to perform ELISA Human - ShhN