Western blot 1,4 β-N-acetyl-D-glucosamine Triticum vulgaris Bacteria

Get tips on using Anti-Caspase-8 antibody (ab25901) to perform Western blotting Caspase-8

Get tips on using Anti-Bcl-2 antibody (ab196495) to perform Western blotting Bcl- 2

Get tips on using E-cadherin Monoclonal Antibody (4A2C7) to perform Western blotting E-cadherin

Get tips on using Anti-TNF alpha antibody (ab6671) to perform Western blotting TNF-alpha

Get tips on using TNFα Antibody (52B83): sc-52746 to perform Western blotting TNF-alpha

Get tips on using Cytochrome C Monoclonal Antibody (37BA11) to perform Western blotting Cytochrome C

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

Get tips on using Vimentin Antibody (E-5): sc-373717 to perform Western blotting Vimentin

Get tips on using Recombinant Anti-PCNA antibody [EPR3821] (ab92552) to perform Western blotting PCNA

Get tips on using COX4 Antibody (F-8): sc-376731 to perform Western blotting COX4