Protein Expression Prokaryotic cells E. coli

Get tips on using VRESelect Medium #63751 to perform Bacterial cell culture media Enterococcus faecium

Get tips on using VRESelect Medium #63751 to perform Bacterial cell culture media Enterococcus faecalis

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse endometrium organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human endometrium organoids

Get tips on using BHI - Brain Heart Infusion to perform Bacterial cell culture media Salmonella enterica

Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Salmonella enterica

Get tips on using BRAIN HEART INFUSION BROTH to perform Bacterial cell culture media Enterococcus faecalis

Get tips on using ATG5 Antibody Novus to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.