An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using REPLI-g Mini Kit (100) to perform Whole Genome Amplification Parasites
Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse iPSCs
Get tips on using QIAamp MinElute Media Kit to perform DNA isolation / purification Tissue - genital / cervical samples
Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse liver tissue
Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse hippocampal tissue
Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse iPSCs
Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - mouse primordial germ cells
Get tips on using QIAamp Media MDx Kit (12) to perform DNA isolation / purification Tissue - genital / cervical samples
Get tips on using REPLI-g Single Cell Kit (96) to perform Whole Genome Amplification Cell lines
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment