DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using EPAS-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HeLa EPAS-1 Lipid

Get tips on using 24-Well Haptotaxis Assays, Collagen I to perform Cell migration / Invasion cell type - HEK293T

Get tips on using Astrocyte Medium to perform Stem cell Differentiation media Glioma differentiation Human astrocytic lineage

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human gastric cancer organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human liver cancer organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Human small intestinal organoids

Get tips on using EpiCult™-B Mouse Medium Kit to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using OCT4-PG1 siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) OCT4-PG1