siRNA / RNAi /miRNA transfection Human Cells HeLa

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Cell cytotoxicity / Proliferation assay cell type - HT22 mouse hippocampal cells

Get tips on using pMHL_2002-bfloGFPa1 to perform Protein Expression Eukaryotic cells - T. pseudonana IbpA DR2 antigen from Histophilus somni

Get tips on using pPICZαB/α-amylase to perform Protein Expression Eukaryotic cells - P. pastoris G. stearothermophilus SR74 α-amylase

Get tips on using pTip-QC2-gi_21222831 to perform Protein Expression Prokaryotic cells - R. erythropolis putative merR-family transcriptional regulator

Get tips on using pTip-QC2-gi_21222214 to perform Protein Expression Prokaryotic cells - R. erythropolis putative araC-family transcriptional regulator

Get tips on using rMaj-pCHH-B to perform Protein Expression Prokaryotic cells - E. coli M. japonicus CHH-like peptide

Get tips on using pTY- α-amylase to perform Protein Expression Prokaryotic cells - E. coli Pyrococcus woesei Hyperthermophile α-Amylase

Get tips on using pST44-yAda3Δ2HIS-yAda2Δ1-yGcn5 to perform Protein Expression Prokaryotic cells - E. coli Ada2/Ada3/Gcn5 complex

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells