RNA sequencing Mouse

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using hCas9 to perform CRISPR Rat - Activation CD2

Get tips on using - to perform siRNA / miRNA gene silencing Rat - IEC-6

Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 SSH1

Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 SSH2

Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 LIMK1

Get tips on using pSUPER.retro.puro to perform shRNA gene silencing Rat - MM1 ADF

Get tips on using SECISBP2 Polyclonal antibody to perform Immunohistochemistry Rat - SBP2

Get tips on using HighCell# ChIP kit to perform ChIP Rat - PCCL3

Get tips on using EZ-ChIP™ to perform ChIP Rat - NRK52E