rna-isolation-purification-cells-primary-mouse-cortical-neurons

Get tips on using pET44a-CrtY to perform Protein Expression Prokaryotic cells - E. coli CrtY

Get tips on using pXMJ19-xynA to perform Protein Expression Prokaryotic cells - C. glutamicum XynA

Get tips on using pAU5-amyE to perform Protein Expression Prokaryotic cells - C. glutamicum amyE

Get tips on using pBNS2-man to perform Protein Expression Prokaryotic cells - B. subtilis mannanase

Get tips on using pBYR2T-EGFP to perform Protein Expression Prokaryotic cells - A. tumefaciens GFP

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using DNase Max Kit (50) to perform Removal of contamination in RNA DNA contamination

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR

Get tips on using pMAPLe3 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex

Get tips on using pMAPLe4 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex