Immunohistochemistry Collagen Type III Goat

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Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Cellular assays Cell migration / Invasion cell type isolated human neutrophils

Get tips on using Anti-Glucocorticoid Receptor antibody (ab3578) to perform Immunohistochemistry Human - GR/glucocorticoid receptor

Products Abcam Anti-Glucocorticoid Receptor antibody (ab3578)

Get tips on using Anti-Mouse CD31 (PECAM-1) to perform Immunohistochemistry CD31 - Rat Mouse -NA-

Products Dianova Anti-Mouse CD31 (PECAM-1)

Get tips on using E-Cadherin (24E10) Rabbit mAb #3195 to perform Immunohistochemistry Human - E-Cadherin

Products Cell Signaling Technology E-Cadherin (24E10) Rabbit mAb #3195

Get tips on using CC10 Antibody (E-11): sc-365992 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Products Santa Cruz Biotechnology CC10 Antibody (E-11): sc-365992

Get tips on using CC10 Antibody (B-6): sc-390313 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Products Santa Cruz Biotechnology CC10 Antibody (B-6): sc-390313

Get tips on using FGF-10 Antibody (3C7): sc-293208 to perform Immunohistochemistry Human - FGF-10

Products Santa Cruz Biotechnology FGF-10 Antibody (3C7): sc-293208

Get tips on using Mucin 5AC Antibody (45M1): sc-21701 to perform Immunohistochemistry Human - Muc-5AC

Products Santa Cruz Biotechnology Mucin 5AC Antibody (45M1): sc-21701

Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2

Products Cell Marque Tissue Diagnostics MUC2 (MRQ-18) Mouse Monoclonal Antibody

Get tips on using Mucin 1 Antibody (VU4H5): sc-7313 to perform Immunohistochemistry Human - Muc-1

Products Santa Cruz Biotechnology Mucin 1 Antibody (VU4H5): sc-7313

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