siRNA / miRNA gene silencing Rat RMC-1

Get tips on using IRF-1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - B16-F10 IRF1

Get tips on using Gβ 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D GNB1

Get tips on using caveolin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B CAV1

Get tips on using ERK 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - WI-38 ERK1

Get tips on using MEK-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - WI-38 MEK1

Get tips on using GLI-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - WI-38 GL1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Slc1a2 to perform siRNA / miRNA gene silencing Rat - Glial cells GLT-1

Get tips on using SNAI 1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - MDA-MB-468 SNAI 1

Get tips on using SNAI 1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - MDA-MB-231 SNAI 1