Site Directed Mutagenesis (SDM) Human Point mutation HUVEC

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using SwaI NEB#R0604 to perform Restriction Enzymes SwaI / SmiI

Get tips on using pLKO5.sgRNA.EFS.GFP to perform CRISPR Mouse - Activation Neuro-2a Smn1

Get tips on using pMyCA‐mCherry to perform Protein Expression Prokaryotic cells - M. smegmatis mCherry

Get tips on using GeneJET Plasmid Miniprep Kit to perform Plasmid Isolation Bacillus smithii

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Mouse Small intestine

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary mouse pulmonary artery smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat pulmonary artery smooth muscle cells