DNA transfection Mammalian cells Immortalized cell lines

Get tips on using LC3A/B Antibody to perform Autophagy assay cell type - A2780

Get tips on using Anti-LC3 Antibody to perform Autophagy assay cell type - MCF7

Get tips on using LC3A/B Antibody to perform Autophagy assay cell type - MCF7

Get tips on using Anti-mTOR antibody to perform Autophagy assay cell type - HepG2

Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - A549

Get tips on using Pan Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Get tips on using Classical Monocyte Isolation Kit, human to perform Cell Isolation Monocyte

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.