Protein Expression Prokaryotic cells B. bifidum

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using BmtI-HF® to perform Restriction Enzymes BmtI

Get tips on using BglII restriction enzyme to perform Restriction Enzymes BglII

Get tips on using BglII NEB#R0144 to perform Restriction Enzymes BglII

Get tips on using BbsI-HF® to perform Restriction Enzymes BbsI

Get tips on using BbsI NEB#R0539 to perform Restriction Enzymes BbsI

Get tips on using BclI NEB#R0160 to perform Restriction Enzymes BclI

Get tips on using BseYI NEB#R0635 to perform Restriction Enzymes BseYI

Get tips on using BmrI NEB#R0600 to perform Restriction Enzymes BmrI

Get tips on using BglI restriction enzyme to perform Restriction Enzymes BglI