Stem cell culture media

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human islets of langerhans

Get tips on using ElectraSense 4x2K/12K to perform Microarray Gene expression arrays - Human whole blood cells Biotin

Get tips on using pGL3-Basic Vector to perform Reporter gene assay luciferase - vascular smooth muscle cells (VSMC)

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using CometChip Electrophoresis Starter Kit to perform DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using Comet SCGE assay kit to perform DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using pPIC9-His-FIP-Ganoderma to perform Protein Expression Eukaryotic cells - P. pastoris Ganoderma FIP

Get tips on using pTip-QC2-gi_21221697 to perform Protein Expression Prokaryotic cells - R. erythropolis putative gntR-family regulator

Get tips on using pTip-QC2-gi_21218674 to perform Protein Expression Prokaryotic cells - R. erythropolis putative DNA-binding protein

Get tips on using pMHL_2002-bfloGFPa1 to perform Protein Expression Eukaryotic cells - T. pseudonana IbpA DR2 antigen from Histophilus somni