rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

Get tips on using pAU5-amyE to perform Protein Expression Prokaryotic cells - C. glutamicum amyE

Get tips on using pBNS2-man to perform Protein Expression Prokaryotic cells - B. subtilis mannanase

Get tips on using pBYR2T-EGFP to perform Protein Expression Prokaryotic cells - A. tumefaciens GFP

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using DNase Max Kit (50) to perform Removal of contamination in RNA DNA contamination

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR

Get tips on using pMAPLe3 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex

Get tips on using pMAPLe4 to perform Protein Expression Prokaryotic cells - E. coli mycobacterial Esx complex

Get tips on using pCcrtB to perform Protein Expression Prokaryotic cells - E. coli Kocuria gwangalliensis CrtB