rna-isolation-purification-cells-primary-porcine-primary-airway-epithelial-cell

Get tips on using QIAGEN Plasmid Plus 96 Miniprep Kit (4) to perform Plasmid Isolation E. coli DH5α

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells

Get tips on using E.Z.N.A.® Plasmid Mini Kit I, (Q-spin) to perform Plasmid Isolation Actinomyces odontolyticus

Get tips on using EZ-10 Spin Column Plasmid DNA Miniprep Kit to perform Plasmid Isolation Cronobacter sakazakii

Get tips on using E.Z.N.A.® Plasmid Mini Kit I, (Q-spin) to perform Plasmid Isolation Salmonella Heidelberg

Get tips on using E.Z.N.A.® Plasmid Mini Kit I, (Q-spin) to perform Plasmid Isolation Bacillus licheniformis

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using LC3B Antibody #2775 to perform Autophagy assay cell type - Mast cell

Get tips on using SPLInsert™ Hanging to perform Cell migration / Invasion cell type - HaCat