Protein Expression Prokaryotic cells B. licheniformis

Get tips on using FbaI (BclI) restriction enzyme to perform Restriction Enzymes BclI

Get tips on using BclI (10 U/µL) to perform Restriction Enzymes BclI

Get tips on using BglI (10 U/µL) to perform Restriction Enzymes BglI

Get tips on using BDNF Rat ELISA Kit to perform ELISA Rat - BDNF

Get tips on using Bambi siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 BAMBI

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using 100 bp DNA Ladders to perform DNA Ladder 100 bp

Get tips on using 10 bp DNA Ruler to perform DNA Ladder 10 bp

Get tips on using 20 bp DNA Ruler to perform DNA Ladder 20 bp

Get tips on using 50 bp DNA Ladder to perform DNA Ladder 50 bp