ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α -NA- Human

Get tips on using HT 8-oxo-dG ELISA Kit II to perform DNA Damage Assay Capan-2

Get tips on using HT 8-oxo-dG ELISA Kit II to perform DNA Damage Assay MIA PaCa-2

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using CD126 antibody | B-R6 to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha

Get tips on using pIL-15 to perform Protein Expression Eukaryotic cells - HEK293 IL-15

Get tips on using PE-Cy™7 Rat Anti-Mouse TNF to perform Flow cytometry Anti-bodies Mouse - TNF-α

Get tips on using GeneArt™ Site-Directed Mutagenesis System to perform Site Directed Mutagenesis (SDM) Human - Point mutation THP-1 IL-33

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using pET20b-chIL-7/H to perform Protein Expression Prokaryotic cells - E. coli chicken IL-7

Get tips on using NA-Star™ Influenza Neuraminidase Inhibitor Resistance Detection Kit to perform Cell Culture Contamination Detection Kit Virus