Immunohistochemistry Alpha smooth muscle Actin Rabbit

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Estrogen Receptor alpha Monoclonal Antibody (SP1) to perform Immunohistochemistry Human - ER

Get tips on using Estrogen Receptor alpha Antibody (F-10): sc-8002 to perform Immunohistochemistry Mouse - ERα

Get tips on using Donkey anti-rabbit IgG to perform Immunohistochemistry Anti-rabbit IgG - Donkey Rabbit Rhodamin red

Get tips on using Alpha Tubulin Antibody to perform Western blotting Tubulin alpha

Get tips on using Recombinant Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (ab32063) to perform Immunohistochemistry Mouse - ERα

Get tips on using pET21a-alpha-synuclein to perform Protein Expression Prokaryotic cells - E. coli alpha-synuclein

Get tips on using HIF-1 alpha Antibody to perform Western blotting HIF-1 alpha

Get tips on using PDGF Receptor β (28E1) Rabbit mAb to perform Immunohistochemistry PDGFβR - Rabbit Mouse -NA-

Get tips on using 53BP1 Antibody (H-300) rabbit polyclonal to perform Immunohistochemistry 53BP1 - Rabbit IgG Human -NA-