Western blot LC3B

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Human melanocytes

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - SAE cells

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - K562 cells

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Mouse embryonic fibroblasts

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Corticospinal motor neurons

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Lens Epithelial Cells

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - HK-2 cells

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Normal human fibroblasts (NHFs)

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.