Immunofluorscence  53BP1 [H-300] Rabbit Human

Get tips on using siGENOME Human CHUK (1147) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - PANC-1 IKKα/CHUK

Get tips on using siGENOME Human IKBKB (3551) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - PANC-1 IKKβ/IKBKB

Get tips on using siGENOME Human GSK3A (2931) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - PANC-1 GSK-3α

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.