Protein Expression Prokaryotic cells E. coli

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Proteins Immunohistochemistry Human E-Cadherin

Get tips on using pMT/BiP/V5-His A, B, & C Drosophila Expression Vectors to perform Protein expression and purification Insect cells - S2 HER2

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Get tips on using E-cadherin Monoclonal Antibody (4A2C7) to perform Western blotting E-cadherin

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Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

Cell culture media Bacterial cell culture media Escherichia coli

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Bacteria - Escherichia coli

Products Thermo Fisher Scientific Y-PER™ Yeast Protein Extraction Reagent

Get tips on using E-cadherin monoclonal antibody (ECCD-2) to perform Western blotting E-cadherin

Products Enzo Life Sciences E-cadherin monoclonal antibody (ECCD-2)

Get tips on using E-Cadherin (24E10) Rabbit mAb #3195 to perform Immunohistochemistry Human - E-Cadherin

Products Cell Signaling Technology E-Cadherin (24E10) Rabbit mAb #3195

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - HOG

Products Thermo Fisher Scientific Subcellular Protein Fractionation Kit for Cultured Cells

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - STTG1

Products Thermo Fisher Scientific Subcellular Protein Fractionation Kit for Cultured Cells

Get tips on using Subcellular Protein Fractionation Kit for Cultured Cells to perform Protein isolation Mammalian cells - Rat_Circumvallate papillae

Products Thermo Fisher Scientific Subcellular Protein Fractionation Kit for Cultured Cells

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