rna-isolation-purification-tissue-mouse-hippocampus

Get tips on using RNeasy Mini Kit to perform

Get tips on using RNeasy Mini Kit to perform

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 ROCK2

Get tips on using RNeasy Plus Mini Kit to perform

Get tips on using RNeasy Plus Mini Kit to perform

Get tips on using RNeasy Plus Mini Kit to perform

Get tips on using RNeasy Plus Mini Kit to perform AAA for reviews

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using Rn_Rab7_5 FlexiTube siRNA(r) to perform siRNA / miRNA gene silencing Rat - NRVM( Rab7

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.