rna-isolation-purification-tissue-mouse-spleen

Get tips on using RNeasy Plus Mini Kit to perform AAA for reviews

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using RCAS1 siRNA (h) to perform siRNA / miRNA gene silencing Human - ES2 RCAS1

Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells hsa-miR-542-3p Lipid

Get tips on using Silencer®_Faslg siRNA (r) to perform siRNA / miRNA gene silencing Rat - F98 Faslg

Get tips on using Silencer® Select_Alkbh1 siRNA (r) to perform siRNA / miRNA gene silencing Rat - B35 Alkbh1

Get tips on using Silencer® Select_Vamp2 siRNA(r) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Vamp2

Get tips on using Silencer® Select_Vamp7 siRNA(r) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Vamp7

Get tips on using Silencer® Select_Mapk14/p38 siRNA(r) to perform siRNA / miRNA gene silencing Rat - NRVM( Mapk14/p38