Protein expression and purification Insect cells Hi5

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Get tips on using SQSTM1/p62 Antibody to perform Autophagy assay cell type - K562 cells

Get tips on using StemSpan™ SFEM to perform Stem cell Differentiation media hiPSCs differentiation into mesodermal lineage cells

Get tips on using Neural Progenitor Medium 2 to perform Stem cell Differentiation media Differentiation of Human PSC into Neural progenitor cells

Get tips on using HIF-1α siRNA (r) to perform siRNA / miRNA gene silencing Rat - Brain endothelial cells HIF-1α Lipid

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human Tendon Stem/Pluripotence cells (TSPCs)

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell Differentiation media hTSPCs differentiation into tenogenic cells

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell Differentiation media hTSPCs differentiation into Chondrogenic cells