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Get tips on using Fluorescent Low Range DNA Ladder to perform DNA Ladder Low Range

Get tips on using Fluorescent Long Range DNA Ladder to perform DNA Ladder Long Range

Get tips on using Fluorescent Mid Range DNA Ladder to perform DNA Ladder Medium Range

Get tips on using Long Range DNA Ladder (linear scale) to perform DNA Ladder Long Range

Get tips on using TriDye™ Ultra Low Range DNA Ladder to perform DNA Ladder Low Range

Get tips on using E-Gel™ Ultra Low Range DNA Ladder to perform DNA Ladder Low Range

Get tips on using E-Gel™ Low Range Quantitative DNA Ladder to perform DNA Ladder Low Range

Get tips on using Rat C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Rat - C-Reactive Protein/CRP

Get tips on using Rat CRP/C Reactive Protein PicoKine™ ELISA Kit to perform ELISA Rat - C-Reactive Protein/CRP

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.