siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

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Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized C-33 A

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized SK-BR-3

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Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized C3H-10T1/2