Protein Expression Eukaryotic cells N. benthamiana

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Hoechst 33342 to perform Necrosis MIA PaCa-2

Get tips on using FastDigest Hin1II to perform Restriction Enzymes Hin1II / NlaIII

Get tips on using FastDigest Mph1103I to perform Restriction Enzymes NsiI / Mph1103I

Get tips on using FastDigest PdiI to perform Restriction Enzymes PdiI / NaeI

Get tips on using Cas9m4-VP64 to perform CRISPR Human - Activation NEUROD1

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion NGRN

Get tips on using MLM3636 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using JDS246 to perform CRISPR Mouse - Deletion Neuro 2a Prnp