DNA transfection Mammalian cells Immortalized cell lines

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - HepG2

Get tips on using APG5 Antibody (C-1) to perform Autophagy assay cell type - A549

Get tips on using Anti-LC3B antibody [EPR18709] to perform Autophagy assay cell type - HepG2

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - HeLa

Get tips on using bisBenzimide H 33342 trihydrochloride to perform Apoptosis assay cell type - OV2008

Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Isolation Kit to perform Cell Isolation Monocyte

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.