CRISPR Mouse Deletion ES (embryonic stem) cells

Get tips on using Mouse Myeloperoxidase DuoSet ELISA to perform ELISA Mouse - MPO

Get tips on using Mouse Leptin ELISA Kit to perform ELISA Mouse - Leptin

Get tips on using Mouse Decorin ELISA Kit to perform ELISA Mouse - Decorin

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human pluripotent stem cells

Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN

Get tips on using Minimum Essential Medium Eagle to perform Stem cell Differentiation media hDPSCs differentiation into chondrogenic cells

Get tips on using Minimum Essential Medium Eagle to perform Stem cell Differentiation media hDPSCs differentiation into osteogenic cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.