Immunohistochemistry Collagen Type III

Get tips on using Anti-alpha smooth muscle Actin antibody (ab5694) to perform Immunohistochemistry Mouse - SMA

Get tips on using Polyclonal Rabbit Anti-Human Myeloperoxidase (Dako Omnis) to perform Immunohistochemistry Mouse - MPO

Get tips on using Anti-UCP-1 antibody produced in rabbit to perform Immunohistochemistry Mouse - UCP1

Get tips on using Anti-CD133 antibody - Stem Cell Marker (ab19898) to perform Immunohistochemistry Mouse - CD133

Get tips on using Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker to perform Immunohistochemistry Mouse - p62

Get tips on using PCNA (D3H8P) XP® Rabbit mAb #13110 to perform Immunohistochemistry Mouse - PCNA

Get tips on using Notch1 (D1E11) XP® Rabbit mAb #3608 to perform Immunohistochemistry Mouse - Notch1

Get tips on using Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb #4147 to perform Immunohistochemistry Mouse - Notch1

Get tips on using Recombinant Anti-Lysozyme antibody [EPR2994(2)] (ab108508) to perform Immunohistochemistry Mouse - Lysozyme

Cell cytotoxicity assays measure the ability of certain compounds or chemical mediators to reduce the viability of the cells. The term cell cytotoxicity assay can sometimes be used interchangeably with cell proliferation assay. Healthy living cells can be identified by the use of formazan dyes, protease biomarkers or by measuring ATP content. The formazan dyes are chromogenic products formed by the reduction of tetrazolium salts by dehydrogenases, such as lactate dehydrogenase (LDH) and reductases that are released during cell death. Common tetrazolium salts include INT, MTT, MTS and XTT. Cell cytotoxicity can also be measured by using the SRB and WST-1 assays. These assays can usually be used in a high-throughput fashion and can be quantitated by measuring absorbance, colorimetry or luminescence. All these assays require similar numbers of cell plating at the initiation, a time course of treatment with the cytotoxic agent and at least triplicates for each condition at every point of analysis. Cell shrinkage, plasma membrane blebbing, cell detachment, externalization of phosphatidylserine, nuclear condensation and ultimately DNA fragmentation are well-described features of apoptosis. The assays that rely on cell membrane integrity for their function, may not be able to quantify early apoptosis. Therefore, in order to distinguish early apoptotic vs. late apoptotic or necrotic cells, additional flow cytometry techniques can be used. A combination of Annexin V and PI (propidium iodide) can be used to distinguish early (Annexin V+/PI-) and late apoptotic (Annexin V+/PI+) cells. Sometimes, caspase assays are used in order to differentiate the stages of apoptosis.