Get tips on using ERK1/2 Antibody to perform Western blotting ERK
Get tips on using ERK 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF ERK1
Get tips on using ERK 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - WI-38 ERK1
Get tips on using ERK 1/2 Antibody (C-9): sc-514302 to perform Western blotting ERK
Get tips on using ON-TARGETplus Human ERCC4 (2072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS XPF/ERCC4
Get tips on using Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse to perform Western blotting ERK
Get tips on using ErbB2 (HER-2) Antibody Cocktail to perform Western blotting HER2
Get tips on using pTRAkc-ERH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
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