siRNA / miRNA gene silencing Human THP-1

Get tips on using Accell Human CD44 (960) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HaCaT CD44

Get tips on using ON-TARGETplus Human EGFR (1956) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - TT EGFR

Get tips on using ON-TARGETplus Human CDK2 (1017) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CDK2

Get tips on using ON-TARGETplus Human IRF9 (10379) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Keratinocytes IRF9

Get tips on using ON-TARGETplus Human CLCN3 (1182) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HEK293 CLCN3

Get tips on using ON-TARGETplus Human CDK9 (1025) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A549 CDK9

Get tips on using ON-TARGETplus Human CYP3A4 (1576) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Primary Human Hepatocytes CYP3A4

Get tips on using ON-TARGETplus Human CYP2B6 (1555) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Primary Human Hepatocytes CYP2B6

Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi has been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining the efficacy of transduction and shRNA on its target site.

Get tips on using Ccl2 siRNA to perform siRNA / miRNA gene silencing Rat - Neuronal cells MCP-1