siRNA / miRNA gene silencing Mouse 3T3-L1

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay .

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Timp2 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 TIMP-2

Get tips on using NFE2L2 siRNA to perform siRNA / miRNA gene silencing Mouse - B16-F10 Nrf2

Get tips on using RORC siRNA-NM_011281 to perform siRNA / miRNA gene silencing Mouse - FL83B Rorc

Get tips on using Mm_Pttg1ip_7 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - AtT20 PTTG1

Get tips on using Mm_Gadd45b_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - AtT20 GADD45β

Get tips on using Cdx2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC Cdx2

Get tips on using BECN1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BV2 BECN1

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Colon26 CD98