Site Directed Mutagenesis (SDM) Human Deletion HepG2

Get tips on using Smad4 Antibody (B-8): sc-7966 to perform Western blotting Smad4

Get tips on using Smad2 Antibody (YZ-13): sc-101153 to perform Western blotting Smad2

Get tips on using Anti-Smad2 (phospho S467) antibody (ab53100) to perform Western blotting Smad2

Get tips on using Smooth Muscle Actin Antibody (B4): sc-53142 to perform Immunohistochemistry Mouse - SMA

Get tips on using Anti-alpha smooth muscle Actin antibody (ab5694) to perform Immunohistochemistry Mouse - SMA

Get tips on using Phospho-SMAD2 (Ser465, Ser467) Monoclonal Antibody (H.205.4) to perform Western blotting Smad2

Get tips on using Anti-alpha smooth muscle Actin antibody to perform Immunohistochemistry Alpha smooth muscle Actin - Rabbit Mouse -NA-

Get tips on using Recombinant Anti-alpha smooth muscle Actin antibody [E184] (ab32575) to perform Western blotting Smooth muscle actin

Get tips on using anti-alpha-Smooth Muscle Actin mouse monoclonal, ASM-1 to perform Immunohistochemistry Alpha smooth muscle Actin - Mouse -NA- -NA-

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.