rna-isolation-purification-cells-primary-porcine-primary-airway-epithelial-cell

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MCF-7

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - SH-SY5Y

Get tips on using LifeGuard Soil Preservation (1000 ml) to perform RNA stabilization Microbial

Get tips on using pBGP3-RsEG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-NtEG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-G1NkBG to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using pBGP3-G1mgNtBG1 to perform Protein Expression Eukaryotic cells - P. pastoris cellulases

Get tips on using gRNA_Cloning Vector to perform CRISPR Mouse - Deletion RMA cells Trh4

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.