rna-isolation-purification-tissue-mouse-cornea

Get tips on using IMAGEN™ Respiratory Syncytial Virus Kit (RSV) using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus

Get tips on using Remel™ PathoDX™ Herpes Typing Kit, PathoDx™ Herpes Typing Kit to perform Cell Culture Contamination Detection Kit Virus

Get tips on using IMAGEN™ Influenza Virus A and B Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus

Get tips on using pcDNA-dCas9-p300 Core to perform CRISPR Human - Activation MASPIN

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using EZchange™ Multi Site-directed Mutagenesis core Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation HEK293 FUS

Get tips on using Unstained Protein Ladder, Broad Range Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained High Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Broad Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Low Range Protein Ladder to perform Protein Ladder Unstained