RNA sequencing

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized Neuro2a

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized DU145

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized Daoy

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized Jurkat

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized A549

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized OVCAR3

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using PureZOL™ RNA Isolation Reagent to perform RNA isolation / purification Tissue - Rat Bone

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized OVCAR-3

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Mammary glands