Get tips on using pGEX-4T-1 to perform Protein Expression Prokaryotic cells - E. coli LsrK
Get tips on using mTeSR™1 to perform Stem cell culture media Human WA09 hESC
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using mTeSR™1 to perform Stem cell culture media hESC lines H9, H1
Get tips on using pPG-1-FlaA to perform Protein Expression Prokaryotic cells - E. coli surface flagellin A
Get tips on using Nectin 1 Monoclonal Antibody (CK8) to perform Flow cytometry Anti-bodies Human - CD111/Nectin-1
Get tips on using siRNA ATX-1 or ENPP2 to perform siRNA / miRNA gene silencing Human - A2780 ATX-1
Get tips on using pET-21b(+)/Pro j 1 to perform Protein Expression Prokaryotic cells - E. coli Pro J 1
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment