Protein Expression Prokaryotic cells B. subtilis

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - K562 cells

Get tips on using EasySep™ Human B Cell Enrichment Kit to perform Cell Isolation B cell

Get tips on using RosetteSep™ Human B Cell Enrichment Cocktail to perform Cell Isolation B cell

Get tips on using EasySep™ Human B Cell Isolation Kit to perform Cell Isolation B cell

Get tips on using Naive B Cell Isolation Kit II, human to perform Cell Isolation Naive B cell

Get tips on using RosetteSep™ HLA B Cell Enrichment Cocktail to perform Cell Isolation HLA B Cell

Get tips on using EasySep™ HLA B Cell Enrichment Kit to perform Cell Isolation HLA B Cell

Get tips on using Anti-LC3A/B antibody (ab62721) to perform Autophagy assay cell type - BRL-3A

Get tips on using Anti-LC3A/B antibody (ab62721) to perform Autophagy assay cell type - PC-12

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.