Protein Expression Prokaryotic cells C. glutamicum

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using APG5 Antibody (C-1) to perform Autophagy assay cell type - A549

Get tips on using Rat Cytochrome c(CYCS) ELISA kit to perform ELISA Rat - Cytochrome c

Get tips on using Cytochrome c Profiling ELISA Kit (ab110172) to perform ELISA Mouse - Cytochrome c

Get tips on using Cytochrome c Profiling ELISA Kit (ab110172) to perform ELISA Rat - Cytochrome c

Get tips on using Human Cytochrome c Quantikine ELISA Kit to perform ELISA Human - Cytochrome C

Get tips on using Cytochrome c Profiling ELISA Kit (ab110172) to perform ELISA Human - Cytochrome C

Get tips on using c-Myc Antibody (9E10): sc-40 to perform Western blotting c-Myc

Get tips on using Lamin A/C Monoclonal Antibody (mab636) to perform Western blotting Lamin A/C

Get tips on using FreeStyle™ 293-F Cells to perform Protein expression and purification Mammalian cells - HEK 293 EGFR