Protein Expression Prokaryotic cells E. coli

Get tips on using EGFR Antibody (E-8): sc-374607 to perform Western blotting EGFR

Get tips on using E-Gel™ Sizing DNA Ladder to perform DNA Ladder Sizing

Get tips on using Vimentin Antibody (E-5): sc-373717 to perform Western blotting Vimentin

Get tips on using HiSpeed Plasmid Midi Kit (25) to perform Plasmid Isolation E. coli

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

Get tips on using CC10 Antibody (E-11): sc-365992 to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using William's E Medium, no phenol red to perform 3D Cell Culture Media Mouse liver organoids

Get tips on using FreeStyle™ 293-F Cells to perform Protein expression and purification Mammalian cells - HEK 293 EGFR

Get tips on using Mouse Integrin alpha E/CD103 Antibody to perform Flow cytometry Anti-bodies Mouse - CD103

Get tips on using Hop Antibody (E-1): sc-398703 to perform Flow cytometry Anti-bodies Mouse - HOPX