rna isolation s.aureus

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RNA isolation / purification Viral - SARS-CoV-2 Experiment

RNA RNA isolation / purification Viral SARS-CoV-2

RNA isolation / purification Supernatant from cell cultures Experiment

RNA RNA isolation / purification Supernatant from cell cultures

RNA isolation / purification Cells - primary mouse morula cells Experiment

RNA RNA isolation / purification Cells primary mouse morula cells

Some help with RNA isolation using Trizol Discussion

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Discussions Some help with RNA isolation using Trizol

RNA isolation / purification Tissue - Livestock Blood / Serum / Plasma / Buffy coat Experiment

RNA RNA isolation / purification Tissue Livestock Blood / Serum / Plasma / Buffy coat

RNA isolation / purification Cells - Cancer cell lines Leukemia cancer cell lines THP-1 Experiment

RNA RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines THP-1

RNA isolation / purification Cells - Cancer cell lines Leukemia cancer cell lines KG-1 Experiment

RNA RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines KG-1

TriPure Isolation Reagent Product

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Rat Pancreas

Products Sigma-Aldrich TriPure Isolation Reagent

TriPure Isolation Reagent Product

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - rat pancreas tissue

Products Sigma-Aldrich TriPure Isolation Reagent

TriPure Isolation Reagent Product

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - Mouse Muscle

Products Sigma-Aldrich TriPure Isolation Reagent

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