The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human mononuclear cells
Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells
Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - rat kidney tissue
Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - rat kidney tissue
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