Protein expression and purification Insect cells S2

Get tips on using Zombie UV™ Fixable Viability Kit to perform Live / Dead assay mammalian cells - mouse splenocytes

Get tips on using Zombie Fixable Viability™ Sampler Kit to perform Live / Dead assay mammalian cells - HEK 293

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay mammalian cells - THP-1

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - adipose stem cells

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - K562 human leukemia cells

Get tips on using Cellular Senescence Flow Cytometry Assay to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Get tips on using Gibco™StemPro™ Adipogenesis Differentiation Kit to perform Stem cell Differentiation media hMSCs differentiation into adipogenic cells

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human primary breast ephitelial cells-organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids