siRNA / RNAi /miRNA transfection Mouse Melanoma cells (B16)

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - HCT-116

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - SW480

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - U266

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - HCT-116

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - HL-60

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - THP-1

Get tips on using Ad-Sal-shRNA to perform shRNA gene silencing Rat - H9c2 salusin-β

Get tips on using Ad-Sal-shRNA to perform shRNA gene silencing Rat - WKY Salusin-β

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using AChE shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 AChE