pcr-hot-start-pcr

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit to perform ROS assay cell type - C2C12

Get tips on using PerCP-Cy5-labeled CD66b to perform FACS CD66b - Mouse Human PerCP-Cy5

Get tips on using PerCP Mouse Anti-Human CD45 to perform Flow cytometry Anti-bodies Human - CD45

Get tips on using CD34 PerCP-Cy™5.5 to perform Flow cytometry Anti-bodies Human - CD34

Get tips on using PerCP Mouse Anti-Human CD19 to perform Flow cytometry Anti-bodies Human - CD19

Get tips on using PerCP Mouse Anti-Human CD14 to perform Flow cytometry Anti-bodies Human - CD14

Get tips on using PerCP Mouse Anti-Human CD3 to perform Flow cytometry Anti-bodies Human - CD3

Get tips on using PerCP Rat Anti-Mouse CD8a to perform Flow cytometry Anti-bodies Mouse - CD8a