siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hMSCs differentiation into osteogenic cells

Get tips on using Gibco™ MEM α, Nucleosides to perform Stem cell Differentiation media hMSCs differentiation into myogenic cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hMSCs differentiation into myogenic cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T

Get tips on using DNeasy Blood and Tissue Kit (250) to perform DNA isolation / purification Cells - Immortalized cell lines Loucy

Get tips on using DMEM with Glucose and L-Glutamine to perform 3D Cell Culture Media U87MG cells- glioblastoma spheres

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.